マウス胚性幹細胞（RF8: 129 SV Jae1系統由来）亜株。Nanog遺伝子の一方がβgeo遺伝子、もう一方がハイグロマイシン耐性遺伝子と置換されている（Nanog遺伝子の転写活性でβgeo遺伝子及びハイグロマイシン耐性遺伝子が発現）。 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach <a href='http://cell.brc.riken.jp/en/wp-content/uploads/restriction/AES0126en.pdf' target="_blank">TERMS and CONDITIONS</a> to MTA (C-0034) . A subline of mouse embryonic stem cell (RF8 derived from 129 SV Jae strain mouse) lacking Nanog expression. Nanog genes were replaced with βgeo gene in one allele and hygromycin resistant gene in another allele. Feeder-free cell line. Nanog^(βgeo/Hyg) 1) 利用者は学術機関に所属している者に限る。2) <a href='http://cell.brc.riken.jp/ja/wp-content/uploads/restriction/AES0126.pdf' target="_blank">別紙1</a>を提供同意書 (C-0028) に添付すること。 <a href="http://www.cira.kyoto-u.ac.jp/" target="_blank">http://www.cira.kyoto-u.ac.jp/</a> Yamanaka, Shinya AES0126